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NeuN Monoclonal antibody, PBS Only

NeuN Monoclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3A4C1

Cat no : 66836-1-PBS

Print datasheet

Synonyms

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

验证数据展示

  • IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66836-1-Ig (same clone as 66836-1-PBS)

    FC experiment of SH-SY5Y using 66836-1-Ig (same clone as 66836-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NeuN (66836-1-Ig, Clone:3A4C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

产品信息

66836-1-PBS targets NeuN in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen NeuN fusion protein Ag28016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexaribonucleotide binding protein 3
Synonyms Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C
GenBank Accession NumberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.