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NeuN Monoclonal antibody, PBS Only

NeuN Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A4C1

Cat No : 66836-1-PBS

Print datasheet

Synonyms

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

验证数据展示

  • IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NeuN antibody included
  • IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

产品信息

66836-1-PBS targets NeuN in IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NeuN fusion protein Ag28016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexaribonucleotide binding protein 3
Synonyms cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3
GenBank Accession NumberNM_001082575
Gene Symbol hCG_1776007
Gene ID (NCBI) 146713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.