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NeutraControl PDGF-BB Monoclonal antibody

NeutraControl PDGF-BB Monoclonal Antibody for ELISA, Non-Neutralization

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

ELISA, Non-Neutralization

Conjugate

Unconjugated

CloneNo.

4E2C7

Cat no : 69520-1-Ig

Print datasheet

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, Proto oncogene c Sis, SIS, SSV

验证数据展示

  • Non-Neutralization experiment of BALB/3T3 cells using 69520-1-Ig

    Non-Neutralization experiment of BALB/3T3 cells using 69520-1-Ig

    Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using 69520-1-Ig

    ELISA experiment of Recombinant protein using 69520-1-Ig

    Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

发表文章中的应用

ELISASee 1 publications below

产品信息

69520-1-Ig targets PDGF-BB in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

Tested Applications ELISA, Non-Neutralization Application Description
Cited ApplicationsELISA
Tested Reactivity Human
Cited Reactivityhuman
Immunogen human Humankine PDGF-BB protein HZ-1308 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
Synonyms Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, Proto oncogene c Sis, SIS, SSV
Gene Symbol PDGFB
Gene ID (NCBI) 5155
RRIDAB_2918854
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

This antibody is a neutralizing control antibody for PDGF-BB, the immunogen is the same as Neutrakine 69020-1-Ig but could not neutralize human PDGF-BB.

发表文章

SpeciesApplicationTitle
humanELISA

Free Radic Biol Med

IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis

Authors - Yao Zhang
View Article