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验证数据展示

  • Non-Neutralization experiment of BALB/3T3 cells using 69520-1-Ig (same clone as 69520-1-PBS)

    Non-Neutralization experiment of BALB/3T3 cells using 69520-1-Ig (same clone as 69520-1-PBS)

    Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69520-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69520-1-Ig (same clone as 69520-1-PBS)

    ELISA experiment of Recombinant protein using 69520-1-Ig (same clone as 69520-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69520-1-PBS in a different storage buffer formulation.

NeutraControl PDGF-BB Monoclonal antibody, PBS Only

NeutraControl PDGF-BB Monoclonal Antibody for ELISA, Non-Neutralization
Cat No. 69520-1-PBS

产品说明书

CloneNo. 4E2C7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

ELISA, Non-Neutralization

别名

PDGFB, PDGFbb, 4E2C7, Becaplermin, PDGF 2

缓冲液配方:  Liquid
Lyophilized
Liquid
偶联物:  Unconjugated
规格价格库存

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产品信息

69520-1-PBS targets PDGF-BB in ELISA, Non-Neutralization applications and shows reactivity with human samples.

经测试应用 ELISA, Non-Neutralization Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human PDGFbb protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
别名 PDGFB, PDGFbb, 4E2C7, Becaplermin, PDGF 2
基因名称 PDGFB
Gene ID (NCBI) 5155
偶联类型 Unconjugated
纯化方式Protein A purification
UNIPROT IDP01127
储存缓冲液 PBS only, pH 7.3.
内毒素<0.1 EU/μg
储存Store at -80°C.

背景介绍

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

This antibody is a neutralizing control antibody for PDGF-BB, the immunogen is the same as Neutrakine 69020-1-Ig but could not neutralize human PDGF-BB.

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