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验证数据展示

  • Non-Neutralization experiment of HUVEC using 69525-1-Ig (same clone as 69525-1-PBS)

    Non-Neutralization experiment of HUVEC using 69525-1-Ig (same clone as 69525-1-PBS)

    Recombinant human VEGF165 (Cat.NO. HZ-1038) stimulates proliferation of HUVEC (human umbilical vein endothelial) cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human VEGF165 (10 ng/mL HZ-1038) is neutralized by mouse anti-human VEGF165/121 monoclonal antibody 69025-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-2 μg/mL. The NeutraControl mouse anti-human VEGF165 antibody 69525-1-Ig could recognize human VEGF165 but could not neutralize human VEGF165 acitivity in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69525-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69525-1-Ig (same clone as 69525-1-PBS)

    ELISA experiment of Recombinant protein using 69525-1-Ig (same clone as 69525-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human VEGF165 (Cat.NO. HZ-1038) and VEGF121 (Cat.NO. HZ-1204) respectively at 70 ng/well followed by blocking and adding serial diluted VEGF165/121 antibody 69025-1-Ig and VEGF165 antibody 69525-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggsts that 69025-1-Ig recognizes both VEGF165 and VEGF121 while 69525-1-Ig recognize VEGF165 only. This data was developed using the same antibody clone with 69525-1-PBS in a different storage buffer formulation.

NeutraControl VEGF165 Monoclonal antibody, PBS Only

NeutraControl VEGF165 Monoclonal Antibody for ELISA, Non-Neutralization
Cat No. 69525-1-PBS

产品说明书

CloneNo. 7E9F4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

ELISA, Non-Neutralization

别名

VEGF, VEGFA, 7E9F4, L-VEGF, N-VEGF

缓冲液配方:  Liquid
Lyophilized
Liquid
偶联物:  Unconjugated
规格价格库存

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69525-1-Ig

Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.

产品信息

69525-1-PBS targets VEGF165 in ELISA, Non-Neutralization applications and shows reactivity with human samples.

经测试应用 ELISA, Non-Neutralization Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human VEGF165 protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, VEGFA, 7E9F4, L-VEGF, N-VEGF
基因名称 VEGFA
Gene ID (NCBI) 7422
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP15692
储存缓冲液 PBS Only, pH 7.4.
内毒素<0.1 EU/μg
储存Store at -80°C.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

This antibody is a neutralizing control antibody for VEGF165, the immunogen is the same as Neutrakine 69025-1-Ig but could not neutralize human VEGF165.

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