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NeutraKine® IL-10 Monoclonal antibody

NeutraKine® IL-10 Monoclonal Antibody for IHC, ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IHC, ELISA, Neutralization and More (1)

Conjugate

Unconjugated

CloneNo.

1E4F5

Cat no : 69018-1-Ig

Print datasheet

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF

验证数据展示

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL.

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using 69018-1-Ig

    ELISA experiment of Recombinant protein using 69018-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • IHC staining of human tonsillitis using 69018-1-Ig

    IHC staining of human tonsillitis using 69018-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 69018-1-Ig

    IHC staining of human tonsillitis using 69018-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Neutralization

产品信息

69018-1-Ig targets NeutraKine® IL-10 in IHC, IF, Neutralization, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA, Neutralization Application Description
Cited ApplicationsIHC, IF, Neutralization
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen human Humankine IL-10 protein HZ-1145 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF
Gene Symbol IL-10
Gene ID (NCBI) 3586
RRIDAB_2918838
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.

实验方案

Product Specific Protocols
IHC protocol for NeutraKine® IL-10 antibody 69018-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Cell Dev Biol

Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway.

Authors - Balun Li
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mouseIHC

Int J Biol Sci

NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.

Authors - Rui Bai
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humanNeutralization,IF

Gut Microbes

Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics

Authors - Li-Wei Xie
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