• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 69018-1-Ig

    Affinity and Kinetic Characterization of 69018-1-Ig

    iolayer interferometry (BLl) kinetic assays of 69018-1-Ig against Human IL-10 were performed. The affinity constant is 0.377 nM.

NeutraKine® IL-10 Monoclonal antibody, PBS Only

NeutraKine® IL-10 Monoclonal Antibody for IHC, Neutralization, ELISA, Indirect ELISA
Cat No. 69018-1-PBS

产品说明书

CloneNo. 1E4F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, Neutralization, ELISA, Indirect ELISA

别名

IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10

缓冲液配方:  PBS Only
Sterile PBS
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

69018-1-PBS targets NeutraKine® IL-10 in IHC, Neutralization, ELISA, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, Neutralization, ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo:

Product name: HumanKine® recombinant human IL-10 protein

Source: -derived,

Tag:

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 10
别名 IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10
基因名称 IL-10
Gene ID (NCBI) 3586
RRIDAB_2918838
偶联类型 Unconjugated
纯化方式Protein G purification
UNIPROT IDP22301
储存缓冲液 PBS only, pH 7.3.
储存Store at -80°C.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.

||
New chat Able
正在加载，请稍候...