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NeutraKine® IL-10 Monoclonal antibody, PBS Only (Capture)

NeutraKine® IL-10 Monoclonal Antibody for IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4F5

Cat no : 69018-1-PBS

Print datasheet

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF

验证数据展示

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

    Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50035-1

    Sandwich ELISA standard curve of MP50035-1

    Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

产品信息

69018-1-PBS targets NeutraKine® IL-10 as part of a matched antibody pair:

MP50035-1: 69018-1-PBS capture and 69018-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen human Humankine IL-10 protein HZ-1145 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 10
Synonyms CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF
Gene Symbol IL-10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.