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NeutraKine® IL-17A Monoclonal antibody, PBS Only (Detector)

NeutraKine® IL-17A Monoclonal Antibody for ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3E3

Cat no : 69021-1-PBS

Print datasheet

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, IL-17A, interleukin 17A, NeutraKine® IL-17A

验证数据展示

  • Neutralization experiment of NeutraKine® IL-17A using 69021-1-Ig (same clone as 69021-1-PBS)

    Neutralization experiment of NeutraKine® IL-17A using 69021-1-Ig (same clone as 69021-1-PBS)

    Recombinant human IL-17A (Cat.NO. HZ-1113) stimulates HT-1080 cells (human fibroblast cell line) produce IL-6 in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human IL-17A (40ng/mL HZ-1113) is neutralized by mouse anti-human IL-17A monoclonal antibody 69021-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 200-600ng/mL. This data was developed using the same antibody clone with 69021-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of NeutraKine® IL-17A using 69021-1-Ig (same clone as 69021-1-PBS)

    Neutralization experiment of NeutraKine® IL-17A using 69021-1-Ig (same clone as 69021-1-PBS)

    Recombinant human IL-17A (Cat.NO. HZ-1113) stimulates HT-1080 cells (human fibroblast cell line) produce IL-6 in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human IL-17A (40ng/mL HZ-1113) is neutralized by mouse anti-human IL-17A monoclonal antibody 69021-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 200-600ng/mL. The NeutraControl mouse anti-human IL-17A monoclonal antibody 69521-1-Ig could recognize human IL-17A, but could not neutralize human IL-17A at the range of 0.3051-5 μg/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69021-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69021-1-Ig (same clone as 69021-1-PBS)

    ELISA experiment of Recombinant protein using 69021-1-Ig (same clone as 69021-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human IL-17A (Cat.NO. HZ-1113) at 70 ng/well followed by blocking and adding serial diluted IL-17A antibody 69021-1-Ig and 69521-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69021-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50004-1

    Sandwich ELISA standard curve of MP50004-1

    Sandwich ELISA standard curve ofMP50004-1, IL17A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69021-2-PBS. Detection antibody: 69021-1-PBS. Standard: HZ-1113. Range: 31.25-2000 pg/mL.

产品信息

69021-1-PBS targets NeutraKine® IL-17A as part of a matched antibody pair:

MP50004-1: 69021-2-PBS capture and 69021-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications ELISA, Sandwich ELISA, Neutralization, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen human Humankine IL-17A protein HZ-1113 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 17A
Synonyms CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, IL-17A, interleukin 17A, NeutraKine® IL-17A
Gene Symbol IL-17A
Gene ID (NCBI) 3605
ENSEMBL Gene IDENSG00000112115
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

This antibody can be used to neutralize the bioactivity of IL-17A.