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验证数据展示

  • Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

    Neutralization experiment of NeutraKine®M-CSF using 69033-1-Ig (same clone as 69033-1-PBS)

    Recombinant human M-CSF(Cat.NO. HZ-1192) stimulates the proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human M-CSF (5 ng/mL) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.4 μg/mL. The control mouse anti-human M-CSF monoclonal antibody 69533-1-Ig could recognize human M-CSF, but could not neutralize human M-CSF at serial does at the range of 0.976563-16000ng/mL(green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 69033-1-Ig (same clone as 69033-1-PBS)

    ELISA experiment of Recombinant protein using 69033-1-Ig (same clone as 69033-1-PBS)

    Indirect ELISA was carried out by coating recombinant Human M-CSF (Cat.NO. HZ-1192) at 70 ng/well followed by blocking and adding serial diluted M-CSF antibody 69033-1-Ig and 69533-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69033-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50033-1

    Sandwich ELISA standard curve of MP50033-1

    Sandwich ELISA standard curve of MP50033-1, MCSF Monoclonal Matched Antibody Pair - PBS Only. 69033-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1192. 68662-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Urine of six individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 1,272.6 pg/mL with a range of 216.3-2,082.1 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Saliva of three individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 185.5 pg/mL with a range of 21.3-473.0 pg/mL.

  • Sample test of MP50033-1

    Sample test of MP50033-1

    Serum of sixteen individual healthy human donors was measured. The M-CSF concentration of detected samples was determined to be 712.6 pg/mL with a range of 223.9-2,041.6 pg/mL.

NeutraKine®M-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine®M-CSF Monoclonal Antibody for Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 69033-1-PBS

产品说明书

CloneNo. 4C6C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test

别名

M-CSF, 4C6C5, CSF 1, CSF1, CSF-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

69033-1-PBS targets NeutraKine®M-CSF as part of a matched antibody pair:

MP50033-1: 69033-1-PBS capture and 68662-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Neutralization, ELISA, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 human HumankineM-CSF protein HZ-1192 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=8.48 x 10-9M
KOff=4.75 x 10-4M
KOn=5.60 x 104M
全称 colony stimulating factor 1 (macrophage)
别名 M-CSF, 4C6C5, CSF 1, CSF1, CSF-1
基因名称 M-CSF
Gene ID (NCBI) 1435
偶联类型 Unconjugated
形式 Lyophilized Powder
纯化方式Protein G purification
储存缓冲液 PBS only , pH 7.3
内毒素<0.1 EU/μg
复溶This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
稳定性与储存Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates the formation of membrane ruffles, cell adhesion, and cell migration, and plays a role in lipoprotein clearance.

This antibody is used to neutralize the bioactivity of M-CSF.