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验证数据展示

  • FC experiment of PC-3 using CL647-69010

    FC experiment of PC-3 using CL647-69010

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Noggin (CL647-69010, Clone:2A11C1) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated Noggin Monoclonal antibody

Noggin Monoclonal Antibody for FC (Intra)
Cat No. CL647-69010

产品说明书

CloneNo. 2A11C1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

NOG, SYNS1, SYM1, 2A11C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 69010-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-69010 targets Noggin in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Noggin fusion protein HZ-1118 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 noggin
别名 NOG, SYNS1, SYM1, 2A11C1
基因名称 Noggin
Gene ID (NCBI) 9241
RRIDAB_2920322
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles. This antibody is used to Block/Neutralize Noggin protein.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Noggin antibody CL647-69010Download protocol
Standard Protocols
Click here to view our Standard Protocols