验证数据展示
经过测试的应用
Positive WB detected in | Y79 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-13497 targets OTX2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | OTX2 fusion protein Ag4323 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | orthodenticle homeobox 2 |
别名 | MCOPS5, Homeobox protein OTX2 |
计算分子量 | 297 aa, 32 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC032579 |
基因名称 | OTX2 |
Gene ID (NCBI) | 5015 |
RRID | AB_3083942 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P32243 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 488 OTX2 antibody CL488-13497 | Download protocol |
FC protocol for CL Plus 488 OTX2 antibody CL488-13497 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |