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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Occludin Polyclonal antibody

Occludin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC and More (1)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13409

Print datasheet

Synonyms

occludin, OCLN

验证数据展示

  • IF Staining of T-47D using CL594-13409

    IF Staining of T-47D using CL594-13409

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:100.

  • IF Staining of MCF-7 using CL594-13409

    IF Staining of MCF-7 using CL594-13409

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Occludin antibody (CL594-13409) at dilution of 1:600.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells, T-47D cells
For other applications, we recommend the unconjugated version of this antibody, 13409-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-13409 targets Occludin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Occludin fusion protein Ag4057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name occludin
Synonyms occludin, OCLN
Calculated Molecular Weight 522 aa, 59 kDa
GenBank Accession NumberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2919795
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16625
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
IF protocol for CL594 Occludin antibody CL594-13409Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Mucosal Immunol

Stress systems exacerbate the inflammatory response after corneal abrasion in sleep-deprived mice via the IL-17 signaling pathway

Authors - Yunxia Xue
View Article