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验证数据展示

  • IF Staining of mouse colon using CL488-11085

    IF Staining of mouse colon using CL488-11085

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

  • IF Staining of mouse colon using CL488-11085

    IF Staining of mouse colon using CL488-11085

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 488 PARD3 antibody (CL488-11085) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PARD3 Polyclonal antibody

PARD3 Polyclonal Antibody for IF-P
Cat No. CL488-11085

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse colon tissue
Planning an IHC experiment? We recommend our IHCeasy PARD3 Ready-To-Use IHC Kit. PARD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11085-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11085 targets PARD3 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 PARD3 fusion protein Ag1565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 par-3 partitioning defective 3 homolog (C. elegans)
别名 ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2
计算分子量 151 kDa
观测分子量 180 kDa, 140-150 kDa, 100 kDa
GenBank蛋白编号BC011711
基因名称 PARD3
Gene ID (NCBI) 56288
RRIDAB_3083892
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEW0
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PARD3 antibody CL488-11085Download protocol
Standard Protocols
Click here to view our Standard Protocols