验证数据展示
经过测试的应用
| Positive WB detected in | A-673 cells, HeLa cells, hTERT-RPE1 cells, JAR cells, K-562 cells, Neuro-2a cells, rat cerebellum tissue, SW480 cells, HT-1376 cells, MG-63 cells, rat retina tissue, mouse retina tissue, rabbit retina tissue |
| Positive IHC detected in | mouse eye tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PAX6 Ready-To-Use IHC Kit. PAX6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 5 publications below |
产品信息
67529-1-Ig targets PAX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | PAX6 fusion protein Ag29977 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | paired box 6 |
| 别名 | Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC011953 |
| 基因名称 | PAX6 |
| Gene ID (NCBI) | 5080 |
| RRID | AB_2882748 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26367 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX6 antibody 67529-1-Ig | Download protocol |
| IHC protocol for PAX6 antibody 67529-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Anal Bioanal Chem Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics. | ||
Stem Cell Res Generation of KCNH2 heterozygous knockout induced pluripotent stem cell (iPSC) line (Long and Short QT Syndrome) | ||
Neural Regen Res Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions | ||
Stem Cell Res Generation of an induced pluripotent stem cell line (HMSCATi004-A) from an early onset Parkinson's disease patient with PRKN gene mutation | ||
Stem Cell Res Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
