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PAX8 Monoclonal antibody, PBS Only

PAX8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B3

Cat no : 60145-4-PBS

Print datasheet

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

验证数据展示

  • IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX8 antibody included
  • IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAX8 antibody included
  • WB analysis of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    WB analysis of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • IF Staining of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    IF Staining of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    IF Staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (60145-4-Ig, Clone: 4H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

产品信息

60145-4-PBS targets PAX8 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name paired box 8
Synonyms PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_10643528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).