验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, human thyroid gland tissue |
| Positive IHC detected in | human ovary tumor tissue, human ovary cancer tissue, human thyroid cancer tissue, human tonsillitis tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60145-4-Ig targets PAX8 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PAX8 fusion protein Ag0306 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | paired box 8 |
| 别名 | PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC001060 |
| 基因名称 | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_10643528 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q06710 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| IHC protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| IF protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition | ||
Mod Pathol Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement. | ||
Stem Cell Res Ther Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells | ||
Front Cell Dev Biol Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway | ||
Hum Pathol Clinicopathological and Molecular Characterization of Biphasic Hyalinizing Psammomatous Renal Cell Carcinoma (BHP RCC): Further Support for the Newly Proposed Entity. |
