• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated PAX8 Monoclonal antibody

PAX8 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H7B3

Cat no : CL488-60145

Print datasheet

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

验证数据展示

  • IF Staining of SKOV-3 using CL488-60145

    IF Staining of SKOV-3 using CL488-60145

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 PAX8 antibody (CL488-60145, Clone: 4H7B3 ) at dilution of 1:800, CL594-Phalloidin (red).

  • IF Staining of human ovary tumor using CL488-60145

    IF Staining of human ovary tumor using CL488-60145

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 PAX8 antibody (CL488-60145, Clone: 4H7B3 ) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman ovary tumor tissue, mouse kidney tissue
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60145 targets PAX8 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name paired box 8
Synonyms PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_2883106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PAX8 antibody CL488-60145Download protocol
Standard Protocols
Click here to view our Standard Protocols