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Biotin-conjugated PAX8 Monoclonal antibody

PAX8 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

4H7B3

Cat no : Biotin-60145

Print datasheet

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

验证数据展示

  • IHC staining of human ovary tumor using Biotin-60145

    IHC staining of human ovary tumor using Biotin-60145

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60145 (PAX8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included
  • IHC staining of human ovary tumor using Biotin-60145

    IHC staining of human ovary tumor using Biotin-60145

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-60145 (PAX8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAX8 antibody included

经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60145 targets PAX8 in IHC applications and shows reactivity with human samples.

Tested Applications IHC Application Description
Tested Reactivity human
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_2883064
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).

实验方案

Product Specific Protocols
IHC protocol for Biotin PAX8 antibody Biotin-60145Download protocol
Standard Protocols
Click here to view our Standard Protocols