验证数据展示
经过测试的应用
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
Biotin-60145 targets PAX8 in IHC applications and shows reactivity with human samples.
Tested Applications | IHC Application Description |
Tested Reactivity | human |
Immunogen | PAX8 fusion protein Ag0306 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | paired box 8 |
Synonyms | paired box 8, Paired box protein Pax 8, PAX8 |
Calculated Molecular Weight | 48 kDa |
GenBank Accession Number | BC001060 |
Gene Symbol | PAX8 |
Gene ID (NCBI) | 7849 |
RRID | AB_2883064 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q06710 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).
实验方案
Product Specific Protocols | |
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IHC protocol for Biotin PAX8 antibody Biotin-60145 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |