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- KD/KO Validated
CoraLite® Plus 488-conjugated PD-L1/CD274 Monoclonal antibody
PD-L1/CD274 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B11D11
验证数据展示
经过测试的应用
| Positive IF detected in | human placenta tissue |
For other applications, we recommend the unconjugated version of this antibody, 66248-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66248 targets PD-L1/CD274 in IF applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Applications | IF-P |
| Tested Reactivity | human, mouse, pig, rat |
| Immunogen | PD-L1/CD274 fusion protein Ag12443 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD274 molecule |
| Synonyms | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1/CD274, Programmed death ligand 1 |
| Calculated Molecular Weight | 290 aa, 33 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC074984 |
| Gene Symbol | CD274 |
| Gene ID (NCBI) | 29126 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NZQ7 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PD-L1/CD274 antibody CL488-66248 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |