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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PDI Polyclonal antibody

PDI Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11245

Print datasheet

Synonyms

DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

验证数据展示

  • IF Staining of HepG2 using CL488-11245

    IF Staining of HepG2 using CL488-11245

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PDI antibody (CL488-11245) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11245-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11245 targets PDI in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen PDI fusion protein Ag1747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prolyl 4-hydroxylase, beta polypeptide
Synonyms DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2919018
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07237
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDI antibody CL488-11245Download protocol
Standard Protocols
Click here to view our Standard Protocols