WB Figures
WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 60225-1-Ig (same clone as 60225-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 60225-1-Ig (same clone as 60225-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)
WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 60225-1-Ig (same clone as 60225-1-PBS)
WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis using 60225-1-Ig (same clone as 60225-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 60225-1-Ig (same clone as 60225-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of Neuro-2a using 60225-1-Ig (same clone as 60225-1-PBS)
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 60225-1-Ig (same clone as 60225-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 60225-1-Ig (same clone as 60225-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of rabbit brain using 60225-1-Ig (same clone as 60225-1-PBS)
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 60225-1-Ig (same clone as 60225-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
WB analysis of rat skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 60225-1-Ig (same clone as 60225-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60225-1-PBS in a different storage buffer formulation.
