- Featured Product
- KD/KO Validated
PI3 Kinase p85 Alpha Monoclonal antibody
PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit and More (3)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
183
CloneNo.
4G3C11
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A549 cells, fetal human brain tissue, Jurkat cells, MCF-10A cells, mouse brain tissue, Neuro-2a cells, PC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, U-251 cells, HT-29 cells, HuT 78 cells, C2C12 cells |
Positive IP detected in | RAW 264.7 cells |
Positive IHC detected in | mouse lung tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60225-1-Ig targets PI3 Kinase p85 Alpha in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP, ELISA |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human, mouse, rat, pig, canine, bovine, duck |
Immunogen | PI3 Kinase p85 Alpha fusion protein Ag2344 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
Synonyms | P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 85 kDa |
GenBank Accession Number | BC030815 |
Gene Symbol | PI3 Kinase p85 Alpha |
Gene ID (NCBI) | 5295 |
RRID | AB_11042594 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P27986 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Autophagy The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis. | ||
Oncogene MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer. | ||
Phytother Res 11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2 /ERβ signaling pathways | ||
Environ Pollut Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats. | ||
Arterioscler Thromb Vasc Biol Short-Term Exposure to Waterpipe/Hookah Smoke Triggers a Hyperactive Platelet Activation State and Increases the Risk of Thrombogenesis. |