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验证数据展示

  • IHC staining of human breast cancer using Biotin-66300

    IHC staining of human breast cancer using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included
  • IHC staining of human ovary tumor using Biotin-66300

    IHC staining of human ovary tumor using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included
  • IHC staining of human breast cancer using Biotin-66300

    IHC staining of human breast cancer using Biotin-66300

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Biotin-66300 (PR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included

Biotin-conjugated PR Monoclonal antibody

PR Monoclonal Antibody for IHC
Cat No. Biotin-66300
CloneNo. 3D1D12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Synonyms

NR3C3, PGR, PR, progesterone receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PR Ready-To-Use IHC Kit. PR primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66300 targets PR in IHC applications and shows reactivity with human samples.

Tested Applications IHC Application Description
Tested Reactivity human
Immunogen PR fusion protein Ag22992 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name progesterone receptor
Synonyms NR3C3, PGR, PR, progesterone receptor
Calculated Molecular Weight 933 aa, 99 kDa
GenBank Accession NumberNM_000926
Gene Symbol Progesterone Receptor
Gene ID (NCBI) 5241
RRIDAB_2883069
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06401
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

实验方案

Product Specific Protocols
IHC protocol for Biotin PR antibody Biotin-66300Download protocol
Standard Protocols
Click here to view our Standard Protocols