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  • Featured Product
  • KD/KO Validated

PRDX4 Monoclonal antibody, PBS Only (Detector)

PRDX4 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B6F1

Cat no : 60286-1-PBS

Print datasheet

Synonyms

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

验证数据展示

  • WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

    WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (60286-1-Ig, Clone: 5B6F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50708-1

    Cytometric bead array standard curve of MP50708-1

    Cytometric bead array standard curve of MP50708-1, PRDX4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60286-2-PBS. Detection antibody: 60286-1-PBS. Standard:Ag13401. Range: 0.391-100 ng/mL.

产品信息

60286-1-PBS targets PRDX4 as part of a matched antibody pair:

MP50708-1: 60286-2-PBS capture and 60286-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRDX4 fusion protein Ag13401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 4
Synonyms Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13162
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).