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  • Featured Product
  • KD/KO Validated

PRDX4 Monoclonal antibody, PBS Only

PRDX4 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5B6F1

Cat No : 60286-1-PBS

Print datasheet

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

验证数据展示

  • WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    WB analysis of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    WB result of PRDX4 antibody (60286-1-Ig, 1:5000) with si-Control and si-PRDX4 transfected HepG2 cells. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

    WB analysis using 60286-1-Ig (same clone as 60286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60286-1-Ig (PRDX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(unde10x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    IHC staining of human colon using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 60286-1-Ig (PRDX4 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    IF Staining of HepG2 using 60286-1-Ig (same clone as 60286-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 60286-1-Ig (PRDX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60286-1-PBS in a different storage buffer formulation.

产品信息

60286-1-PBS targets PRDX4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen PRDX4 fusion protein Ag13401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 4
Synonyms Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13162
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).