验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, K-562 cells, PC-12 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human colon tissue, human pancreas cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60286-1-Ig targets PRDX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PRDX4 fusion protein Ag13401 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | peroxiredoxin 4 |
| 别名 | Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2 |
| 计算分子量 | 31 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC007107 |
| 基因名称 | PRDX4 |
| Gene ID (NCBI) | 10549 |
| RRID | AB_2881403 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13162 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRDX4 antibody 60286-1-Ig | Download protocol |
| IHC protocol for PRDX4 antibody 60286-1-Ig | Download protocol |
| IF protocol for PRDX4 antibody 60286-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma
| ||
Biomolecules Exosome-Related FTCD Facilitates M1 Macrophage Polarization and Impacts the Prognosis of Hepatocellular Carcinoma | ||
Biochem Biophys Res Commun Peroxiredoxin 4 secreted by cumulus cells ameliorates the maturation of oocytes in vitro |
