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验证数据展示

  • IF Staining of HepG2 using CL488-60286

    IF Staining of HepG2 using CL488-60286

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60286 (PRDX4 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PRDX4 Monoclonal antibody

PRDX4 Monoclonal Antibody for IF/ICC
Cat No. CL488-60286

产品说明书

CloneNo. 5B6F1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC

别名

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX4 Ready-To-Use IHC Kit. PRDX4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60286-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60286 targets PRDX4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 PRDX4 fusion protein Ag13401 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 4
别名 Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372
计算分子量 31 kDa
观测分子量 30 kDa
GenBank蛋白编号BC007107
基因名称 PRDX4
Gene ID (NCBI) 10549
RRIDAB_2883134
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13162
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRDX4 antibody CL488-60286Download protocol
Standard Protocols
Click here to view our Standard Protocols