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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRDX4 Monoclonal antibody

PRDX4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B6F1

Cat no : CL488-60286

Print datasheet

Synonyms

Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372

验证数据展示

  • IF Staining of HepG2 using CL488-60286

    IF Staining of HepG2 using CL488-60286

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60286 (PRDX4 antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRDX4 Ready-To-Use IHC Kit. PRDX4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60286-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60286 targets PRDX4 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen PRDX4 fusion protein Ag13401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 4
Synonyms Antioxidant enzyme AOE372, AOE37 2, peroxiredoxin 4, Peroxiredoxin IV, PRDX4, PRX 4, Prx IV, Thioredoxin peroxidase AO372
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007107
Gene Symbol PRDX4
Gene ID (NCBI) 10549
RRIDAB_2883134
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13162
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRDX4 antibody CL488-60286Download protocol
Standard Protocols
Click here to view our Standard Protocols