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验证数据展示

  • IF Staining of mouse brain using CL488-20665

    IF Staining of mouse brain using CL488-20665

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PSD95-Specific,DLG4 antibody (CL488-20665) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PSD95-Specific,DLG4 Polyclonal antibody

PSD95-Specific,DLG4 Polyclonal Antibody for IF-P
Cat No. CL488-20665

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 20665-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20665 targets PSD95-Specific,DLG4 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large homolog 4 (Drosophila)
Synonyms Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberNM_001365
Gene Symbol PSD95
Gene ID (NCBI) 1742
RRIDAB_3084038
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78352
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSD95-Specific,DLG4 antibody CL488-20665Download protocol
Standard Protocols
Click here to view our Standard Protocols