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Phospho-JNK (Tyr185) Recombinant antibody, PBS Only

Phospho-JNK (Tyr185) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, FC (Intra), Indirect ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2G8

Cat no : 80024-1-PBS

Print datasheet

Synonyms

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

验证数据展示

  • WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

    FC experiment of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR, Clone:2G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

80024-1-PBS targets Phospho-JNK (Tyr185) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 8
Synonyms JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24
Observed Molecular Weight37-46 kDa
GenBank Accession NumberNM_139049
Gene Symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2882943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45983
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

发表文章

SpeciesApplicationTitle
human,ratWB,IHC

Redox Rep

Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells

Authors - Xiaozhe Su
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