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Phospho-RIPK1 (Ser161) Monoclonal antibody, PBS Only

Phospho-RIPK1 (Ser161) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B2G1

Cat no : 66854-1-PBS

Print datasheet

Synonyms

RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1

验证数据展示

  • WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    WB analysis using 66854-1-Ig (same clone as 66854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was untreated (left) or treated with Lambda Protein Phosphatase (λ-PPase, 500U, right) at 37℃ for 1 hours before blocking. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    IHC staining of human small intestine using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 66854-1-Ig (same clone as 66854-1-PBS)

    FC experiment of HEK-293T using 66854-1-Ig (same clone as 66854-1-PBS)

    1X10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Anti-Human Phospho-RIPK1 (Ser161) (66854-1-Ig, Clone:1B2G1) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 66854-1-Ig (same clone as 66854-1-PBS)

    IF Staining of U2OS using 66854-1-Ig (same clone as 66854-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66854-1-PBS in a different storage buffer formulation.

产品信息

66854-1-PBS targets Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 74 kDa, 45 kDa
GenBank Accession NumberNM_003804
Gene Symbol RIPK1
Gene ID (NCBI) 8737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13546
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).