验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells |
| Positive IHC detected in | human small intestine tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000- 1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| 别名 | p RIPK1, p RIPK1 (Ser161), p RIPK1 Ser161, Phospho RIPK1, Phospho RIPK1 (Ser161) |
| 计算分子量 | 76 kDa |
| 观测分子量 | 74 kDa, 45 kDa |
| GenBank蛋白编号 | NM_003804 |
| 基因名称 | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_2882194 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13546 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
| IHC protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
| IF protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway | ||
Cell Commun Signal IRF1 regulation of ZBP1 links mitochondrial DNA and chondrocyte damage in osteoarthritis | ||
Inflammation Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected Macrophages | ||
J Cell Mol Med LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis. | ||
BMC Complement Med Ther JianPiYiShen formula prevents cisplatin-induced acute kidney injury in mice by improving necroptosis through MAPK pathway | ||
Front Genet Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation. |
