验证数据展示
经过测试的应用
Positive IF/ICC detected in | Calyculin A treated HeLa cells, U2OS cells |
Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66854 targets Phospho-RIPK1 (Ser161) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
别名 | RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1 |
计算分子量 | 76 kDa |
观测分子量 | 74 kDa, 45 kDa |
GenBank蛋白编号 | NM_003804 |
基因名称 | RIPK1 |
Gene ID (NCBI) | 8737 |
RRID | AB_2919380 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q13546 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Phospho-RIPK1 (Ser161) antibody CL488-66854 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |