CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser161) Monoclonal antibody
Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B2G1
验证数据展示
经过测试的应用
Positive IF detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 66854-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66854 targets Phospho-RIPK1 (Ser161) in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
Synonyms | Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161) , Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1 |
Calculated Molecular Weight | 76 kDa |
Observed Molecular Weight | 74 kDa, 45 kDa |
GenBank Accession Number | NM_003804 |
Gene Symbol | RIPK1 |
Gene ID (NCBI) | 8737 |
RRID | AB_2919380 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q13546 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Phospho-RIPK1 (Ser161) antibody CL488-66854 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |