验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, 4T1 cells, NIH/3T3 cells, K-562 cells, HSC-T6 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAD51 Ready-To-Use IHC Kit. RAD51 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 3 publications below |
产品信息
67024-1-Ig targets RAD51 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | RAD51 fusion protein Ag7410 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) |
| Synonyms | RAD51 homolog A, HsT16930, HsRad51, HRAD51, BRCC5 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC001459 |
| Gene Symbol | RAD51 |
| Gene ID (NCBI) | 5888 |
| RRID | AB_2882339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06609 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAD51 antibody 67024-1-Ig | Download protocol |
| IHC protocol for RAD51 antibody 67024-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles. | ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer | ||
J Med Chem Rational Design of PARP1/c-Met Dual Inhibitors for Overcoming PARP1 Inhibitor Resistance Induced by c-Met Overexpression | ||
Cell Death Dis Iron promotes ovarian cancer malignancy and advances platinum resistance by enhancing DNA repair via FTH1/FTL/POLQ/RAD51 axis | ||
Mol Cancer NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer |
