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  • Featured Product
  • KD/KO Validated

RAD51 Monoclonal antibody, PBS Only

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4A12B8

Cat no : 67024-1-PBS

Print datasheet

Synonyms

BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA

验证数据展示

  • WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

    WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

    IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

    WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

    WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

产品信息

67024-1-PBS targets RAD51 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RAD51 fusion protein Ag7410 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Synonyms BRCC5, HRAD51, HsRad51, HsT16930, RAD51, RAD51 homolog A, RAD51A, RECA
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001459
Gene Symbol RAD51
Gene ID (NCBI) 5888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06609
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.