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验证数据展示

  • WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

    WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

    IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAD51 antibody included
  • WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

    WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

    WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RAD51 Monoclonal antibody, PBS Only

RAD51 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67024-1-PBS

产品说明书

CloneNo. 4A12B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

4A12B8, BRCC5, HRAD51, HsRad51, HsT16930

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAD51 Ready-To-Use IHC Kit. RAD51 primary antibody included.

产品信息

67024-1-PBS targets RAD51 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAD51 fusion protein Ag7410 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
别名 4A12B8, BRCC5, HRAD51, HsRad51, HsT16930
计算分子量 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC001459
基因名称 RAD51
Gene ID (NCBI) 5888
RRIDAB_2882339
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06609
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.