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验证数据展示

  • WB analysis using CL488-17250

    WB analysis using CL488-17250

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-17250 (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RB1CC1 Polyclonal antibody

RB1CC1 Polyclonal Antibody for WB
Cat No. CL488-17250

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB

别名

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy RB1CC1 Ready-To-Use IHC Kit. RB1CC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17250-1-AP

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17250 targets RB1CC1 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 RB1CC1 fusion protein Ag10508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RB1-inducible coiled-coil 1
别名 RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa
计算分子量 1594 aa, 183 kDa
观测分子量 200 kDa
GenBank蛋白编号BC017556
基因名称 RB1CC1
Gene ID (NCBI) 9821
RRIDAB_3084001
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDY2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 RB1CC1 antibody CL488-17250Download protocol
Standard Protocols
Click here to view our Standard Protocols