验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, human spleen tissue, human testis tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human brain tissue, human lung tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
16518-1-AP targets RNASEH2C in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RNASEH2C fusion protein Ag9739 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribonuclease H2, subunit C |
| 别名 | AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C |
| 计算分子量 | 164 aa, 18 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC023588 |
| 基因名称 | RNASEH2C |
| Gene ID (NCBI) | 84153 |
| RRID | AB_2181648 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDP1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEH2C antibody 16518-1-AP | Download protocol |
| IHC protocol for RNASEH2C antibody 16518-1-AP | Download protocol |
| IP protocol for RNASEH2C antibody 16518-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. | ||
Hum Mol Genet Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability. | ||
J Biol Chem Analysis of ribonucleotide removal from DNA by human nucleotide excision repair. | ||
Cell Rep Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition
|
