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验证数据展示

  • IF Staining of A431 using CL488-66227

    IF Staining of A431 using CL488-66227

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®®488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of Saos-2 using CL488-66227

    IF Staining of Saos-2 using CL488-66227

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using CoraLite® Plus 488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for IF/ICC
Cat No. CL488-66227

Print datasheet

CloneNo. 1B3F9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells, Saos-2 cells
Planning an IHC experiment? We recommend our IHCeasy S100A10 Ready-To-Use IHC Kit. S100A10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66227-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66227 targets S100A10 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen S100A10 fusion protein Ag23596 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein A10
Synonyms 42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10
Calculated Molecular Weight 11 kDa
GenBank Accession NumberBC015973
Gene Symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2919303
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60903
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S100A10 antibody CL488-66227Download protocol
Standard Protocols
Click here to view our Standard Protocols