- Featured Product
- KD/KO Validated
S100A14 Polyclonal antibody
S100A14 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, rat stomach tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human ovary tumor tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10489-1-AP targets S100A14 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | S100A14 fusion protein Ag0757 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | S100 calcium binding protein A14 |
| Synonyms | Protein S100 A14, S100A14, S100A15, S114 |
| Calculated Molecular Weight | 12 kDa |
| Observed Molecular Weight | 10-12 kDa |
| GenBank Accession Number | BC005019 |
| Gene Symbol | S100A14 |
| Gene ID (NCBI) | 57402 |
| RRID | AB_2183628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S100A14 antibody 10489-1-AP | Download protocol |
| IHC protocol for S100A14 antibody 10489-1-AP | Download protocol |
| IF protocol for S100A14 antibody 10489-1-AP | Download protocol |
| IP protocol for S100A14 antibody 10489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1. | ||
Int J Cancer S100A14 is a novel independent prognostic biomarker in the triple-negative breast cancer subtype. | ||
Cell Death Dis Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer. | ||
Am J Cancer Res A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction. | ||
Eur J Cancer S100A14 regulates the invasive potential of oral squamous cell carcinoma derived cell-lines in vitro by modulating expression of matrix metalloproteinases, MMP1 and MMP9.
|
