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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated S100A14 Polyclonal antibody

S100A14 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10489

Print datasheet

Synonyms

Protein S100 A14, S100A14, S100A15, S114

验证数据展示

  • IF Staining of HeLa using CL488-10489

    IF Staining of HeLa using CL488-10489

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 S100A14 antibody (CL488-10489) at dilution of 1:200.

  • FC experiment of HeLa using CL488-10489

    FC experiment of HeLa using CL488-10489

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human S100A14 (CL488-10489) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100A14 Ready-To-Use IHC Kit. S100A14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10489-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10489 targets S100A14 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen S100A14 fusion protein Ag0757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A14
Synonyms Protein S100 A14, S100A14, S100A15, S114
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 10-12 kDa
GenBank Accession NumberBC005019
Gene Symbol S100A14
Gene ID (NCBI) 57402
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCY8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S100A14 antibody CL488-10489Download protocol
FC protocol for CL Plus 488 S100A14 antibody CL488-10489Download protocol
Standard Protocols
Click here to view our Standard Protocols