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验证数据展示

  • WB analysis of HeLa using 10245-1-AP

    WB analysis of HeLa using 10245-1-AP

    WB result of S100A6 antibody (10245-1-AP, 1:30000) with si-Control and si-S100A6 transfected HeLa cells..

  • WB analysis using 10245-1-AP

    WB analysis using 10245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10245-1-AP (S100A6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 10245-1-AP

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included
  • IHC staining of human stomach cancer using 10245-1-AP

    IHC staining of human stomach cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10245-1-AP (S100A6 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included
  • IHC staining of human pancreas cancer using 10245-1-AP

    IHC staining of human pancreas cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included
  • IHC staining of human pancreas cancer using 10245-1-AP

    IHC staining of human pancreas cancer using 10245-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10245-1-AP (S100A6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A6 antibody included
  • IF Staining of MCF-7 using 10245-1-AP

    IF Staining of MCF-7 using 10245-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using S100A6 antibody (10245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 10245-1-AP

    FC experiment of MCF-7 using 10245-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human S100A6 (10245-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IP experiment of A549 using 10245-1-AP

    IP experiment of A549 using 10245-1-AP

    IP result of anti-S100A6 (IP:10245-1-AP, 4ug; Detection:10245-1-AP 1:5000) with A549 cells lysate 840 ug.

  • Featured Product
  • KD/KO验证

S100A6 Polyclonal antibody

S100A6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10245-1-AP

产品说明书

引用文献(20)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

PRA, MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A6 Ready-To-Use IHC Kit. S100A6 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10245-1-AP targets S100A6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat, monkey
免疫原

CatNo: Ag0385

Product name: Recombinant human S100A6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC001431

Sequence: MACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSKLQDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein A6
别名 PRA, MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC001431
基因名称 S100A6
Gene ID (NCBI) 6277
RRIDAB_2183801
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06703
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

实验方案

Product Specific Protocols
WB protocol for S100A6 antibody 10245-1-APDownload protocol
IHC protocol for S100A6 antibody 10245-1-APDownload protocol
IF protocol for S100A6 antibody 10245-1-APDownload protocol
IP protocol for S100A6 antibody 10245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell

Cellular architecture of human brain metastases.

Authors - Hugo Gonzalez
View Article
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
View Article
humanIHC,IF

Sci Transl Med

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

Authors - Bradley N Smith
View Article
human,mouseWB,IHC,IF

Cancer Lett

S100A6 drives lymphatic metastasis of liver cancer via activation of the RAGE/NF-kB/VEGF-D pathway

Authors - TianYi Chen
View Article
humanIF

iScience

Lineage tracing reveals transient phenotypic adaptation of tubular cells during acute kidney injury

Authors - Marc Buse
View Article
humanIF

Cancer Commun (Lond)

Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.

Authors - Chaoji Shi
View Article