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验证数据展示

  • FC experiment of MCF-7 using CL488-10245

    FC experiment of MCF-7 using CL488-10245

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human S100A6 (CL488-10245) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of MCF-7 using CL488-10245

    IF Staining of MCF-7 using CL488-10245

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite® Plus 488 S100A6 antibody (CL488-10245) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated S100A6 Polyclonal antibody

S100A6 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10245

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

PRA, MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A6 Ready-To-Use IHC Kit. S100A6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10245-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

CL488-10245 targets S100A6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Cited Reactivityhuman
Immunogen S100A6 fusion protein Ag0385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A6
Synonyms PRA, MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY
Calculated Molecular Weight 10 kDa
GenBank Accession NumberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2918966
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06703
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S100A6 antibody CL488-10245Download protocol
FC protocol for CL Plus 488 S100A6 antibody CL488-10245Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Heliyon

Upregulation of S100A6 and its relation with CD34+ cells apoptosis in high-risk myelodysplastic syndromes patients

Authors - Yan Zhai
View Article