验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells, HepG2 cells, K-562 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Septin 2 fusion protein Ag1946 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | septin 2 |
别名 | NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5 |
计算分子量 | 41 kDa |
观测分子量 | 42-45 kDa |
GenBank蛋白编号 | BC014455 |
基因名称 | Septin 2 |
Gene ID (NCBI) | 4735 |
RRID | AB_2883416 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q15019 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Septin 2 antibody CL594-60075 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |