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验证数据展示

  • IF Staining of HepG2 using CL594-60075

    IF Staining of HepG2 using CL594-60075

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-60075

    IF Staining of MCF-7 using CL594-60075

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • IF Staining of K-562 using CL594-60075

    IF Staining of K-562 using CL594-60075

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for IF/ICC
Cat No. CL594-60075

Print datasheet

CloneNo. 2F3B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HepG2 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN2 Ready-To-Use IHC Kit. SEPTIN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60075-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Septin 2 fusion protein Ag1946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 2
Synonyms NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC014455
Gene Symbol Septin 2
Gene ID (NCBI) 4735
RRIDAB_2883416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15019
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

实验方案

Product Specific Protocols
IF protocol for CL594 Septin 2 antibody CL594-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols