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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SPHK2 Polyclonal antibody

SPHK2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17096

Print datasheet

Synonyms

SPK-2, SPK 2, Sphingosine kinase 2, SK-2, SK 2

验证数据展示

  • FC experiment of HeLa using CL488-17096

    FC experiment of HeLa using CL488-17096

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SPHK2 (CL488-17096) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SPHK2 Ready-To-Use IHC Kit. SPHK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17096-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17096 targets SPHK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SPHK2 fusion protein Ag10569 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingosine kinase 2
Synonyms SPK-2, SPK 2, Sphingosine kinase 2, SK-2, SK 2
Calculated Molecular Weight 618 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010671
Gene Symbol SPHK2
Gene ID (NCBI) 56848
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRA0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SPHK2 antibody CL488-17096Download protocol
Standard Protocols
Click here to view our Standard Protocols