验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse testis tissue, rat lung tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14157-1-AP targets KRAP/SSFA2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, canine |
| 免疫原 | KRAP/SSFA2 fusion protein Ag5339 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sperm specific antigen 2 |
| 别名 | SSFA2, ITPRID2, CS-1, CS1, CS 1 |
| 计算分子量 | 138 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC028706 |
| 基因名称 | SSFA2 |
| Gene ID (NCBI) | 6744 |
| RRID | AB_2195472 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28290 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| IHC protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| IF protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| IP protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.
| ||
J Cell Sci Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.
| ||
J Proteomics Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma. | ||
Reprod Biol Endocrinol A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation. | ||
Front Immunol Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas | ||
J Cell Sci CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors |
