验证数据展示
经过测试的应用
Positive IF-P detected in | mouse pancreas tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-14157 targets KRAP/SSFA2 in IF-P applications and shows reactivity with human, rat, mouse samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, rat, mouse |
免疫原 | KRAP/SSFA2 fusion protein Ag5339 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sperm specific antigen 2 |
别名 | Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2 |
计算分子量 | 138 kDa |
GenBank蛋白编号 | BC028706 |
基因名称 | SSFA2 |
Gene ID (NCBI) | 6744 |
RRID | AB_2883405 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P28290 |
储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 KRAP/SSFA2 antibody CL594-14157 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |