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  • Featured Product
  • KD/KO Validated

STAT3 Monoclonal antibody, PBS Only

STAT3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2D12

Cat No : 60199-1-PBS

Print datasheet

Synonyms

Acute phase response factor, APRF, HIES, STAT3

验证数据展示

  • WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

    WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

    WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

    IP experiment of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

    IP result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

    IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT3 antibody included
  • IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

    IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

  • WB analysis using 60199-1-Ig (same clone as 60199-1-PBS)

    WB analysis using 60199-1-Ig (same clone as 60199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

    IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT3 antibody (60199-1-Ig, Clone: 3G2D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

产品信息

60199-1-PBS targets STAT3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IP, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen STAT3 fusion protein Ag0360 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms Acute phase response factor, APRF, HIES, STAT3
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight 70-88 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40763
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.