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CoraLite®555-conjugated STAT3 Monoclonal antibody

STAT3 Monoclonal Antibody for FC (Intra)
Cat No. CL555-60199

产品说明书

CloneNo. 3G2D12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

STAT3, 3G2D12, Acute-phase response factor, APRF, HIES, STAT 3

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60199-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-60199 targets STAT3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 STAT3 fusion protein Ag0360 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 signal transducer and activator of transcription 3 (acute-phase response factor)
别名 STAT3, 3G2D12, Acute-phase response factor, APRF, HIES, STAT 3
计算分子量 770 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC000627
基因名称 STAT3
Gene ID (NCBI) 6774
RRIDAB_2919659
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40763
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

实验方案

Product Specific Protocols
FC protocol for CL555 STAT3 antibody CL555-60199Download protocol
Standard Protocols
Click here to view our Standard Protocols
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