• Featured Product
  • KD/KO验证

Stathmin 1 Polyclonal antibody

Stathmin 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, PC-12 cells
Positive IHC detected inhuman gliomas tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STMN1 Ready-To-Use IHC Kit. STMN1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11157-1-AP targets Stathmin 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 Stathmin 1 fusion protein Ag1633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stathmin 1/oncoprotein 18
别名 STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC014353
基因名称 Stathmin 1
Gene ID (NCBI) 3925
RRIDAB_2197114
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

实验方案

Product Specific Protocols
WB protocol for Stathmin 1 antibody 11157-1-APDownload protocol
IHC protocol for Stathmin 1 antibody 11157-1-APDownload protocol
IF protocol for Stathmin 1 antibody 11157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Cell Stem Cell

Continuous clonal labeling reveals uniform progenitor potential in the adult exocrine pancreas.

Authors - Sophie C Lodestijn
ratWB

Chemosphere

Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.

Authors - Mingdan You
humanWB

Int J Nanomedicine

Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.

Authors - Chen Li
humanWB

Oncogene

KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.

Authors - Lei Zhao
  • KD Validated
humanIHC

Oncotarget

Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients.

Authors - Xia-Ying Kuang
humanIHC

Neuroimage

Developmental dynamics of the periventricular parietal crossroads of growing cortical pathways in the fetal brain - In vivo fetal MRI with histological correlation.

Authors - Ruxandra-Iulia Milos
Loading...