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验证数据展示

  • FC experiment of HeLa using CL488-11157

    FC experiment of HeLa using CL488-11157

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Stathmin 1 (CL488-11157) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Stathmin 1 Polyclonal antibody

Stathmin 1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-11157

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

C1orf215, FLJ32206, Lag, LAP18, Metablastin, Oncoprotein 18, OP18, Phosphoprotein p19, PP17, PP19, PR22, Prosolin, Protein Pr22, SMN, Stathmin, Stathmin 1, stathmin 1/oncoprotein 18, STMN1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STMN1 Ready-To-Use IHC Kit. STMN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11157-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11157 targets Stathmin 1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Stathmin 1 fusion protein Ag1633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stathmin 1/oncoprotein 18
Synonyms C1orf215, FLJ32206, Lag, LAP18, Metablastin, Oncoprotein 18, OP18, Phosphoprotein p19, PP17, PP19, PR22, Prosolin, Protein Pr22, SMN, Stathmin, Stathmin 1, stathmin 1/oncoprotein 18, STMN1
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC014353
Gene Symbol Stathmin 1
Gene ID (NCBI) 3925
RRIDAB_3672485
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16949
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Stathmin 1 antibody CL488-11157Download protocol
Standard Protocols
Click here to view our Standard Protocols