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  • Featured Product
  • KD/KO Validated

HRD1/SYVN1 Polyclonal antibody

HRD1/SYVN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13473-1-AP

Print datasheet

Synonyms

SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27

验证数据展示

  • WB analysis using 13473-1-AP

    WB analysis using 13473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13473-1-AP

    WB analysis of HeLa using 13473-1-AP

    WB result of HRD1/SYVN1 antibody (13473-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells.

  • IHC staining of human kidney using 13473-1-AP

    IHC staining of human kidney using 13473-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 13473-1-AP

    IHC staining of human kidney using 13473-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 40x lens).

  • IP experiment of mouse kidney using 13473-1-AP

    IP experiment of mouse kidney using 13473-1-AP

    IP result of anti-HRD1/SYVN1 (IP:13473-1-AP, 4ug; Detection:13473-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.

  • IF Staining of HepG2 using 13473-1-AP

    IF Staining of HepG2 using 13473-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HRD1/SYVN1 antibody (13473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, mouse kidney tissue, mouse ovary tissue, mouse pancreas tissue, mouse stomach tissue, NIH/3T3 cells, Raji cells, rat stomach tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13473-1-AP targets HRD1/SYVN1 in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, IP-MS
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen HRD1/SYVN1 fusion protein Ag4267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synovial apoptosis inhibitor 1, synoviolin
Synonyms SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68-76 kDa
GenBank Accession NumberBC030530
Gene Symbol HRD1
Gene ID (NCBI) 84447
RRIDAB_2287023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

实验方案

Product Specific Protocols
WB protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IHC protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IF protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
IP protocol for HRD1/SYVN1 antibody 13473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
View Article
humanIP,WB

J Clin Invest

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Authors - Huilun Helen Wang
View Article
humanWB,IF,IP,IP-MS

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
View Article
humanWB

Mol Cell

Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.

Authors - Annemieke T van der Goot
  • KD Validated
View Article

Mol Cell

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Authors - Corey N Cunningham
View Article
humanWB

Nat Commun

PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.

Authors - Yu-Jie Chen
View Article