验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MDA-MB-453s cells, mouse kidney tissue, mouse ovary tissue, mouse pancreas tissue, mouse stomach tissue, NIH/3T3 cells, Raji cells, rat stomach tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13473-1-AP targets HRD1/SYVN1 in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, IP-MS |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HRD1/SYVN1 fusion protein Ag4267 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synovial apoptosis inhibitor 1, synoviolin |
| 别名 | SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27 |
| 计算分子量 | 617 aa, 68 kDa |
| 观测分子量 | 68-76 kDa |
| GenBank蛋白编号 | BC030530 |
| 基因名称 | HRD1 |
| Gene ID (NCBI) | 84447 |
| RRID | AB_2287023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86TM6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRD1/SYVN1 antibody 13473-1-AP | Download protocol |
| IHC protocol for HRD1/SYVN1 antibody 13473-1-AP | Download protocol |
| IF protocol for HRD1/SYVN1 antibody 13473-1-AP | Download protocol |
| IP protocol for HRD1/SYVN1 antibody 13473-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. | ||
J Clin Invest Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders | ||
Mol Cancer CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. | ||
Mol Cell Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.
| ||
Nat Commun PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones. |
