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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HRD1/SYVN1 Polyclonal antibody

HRD1/SYVN1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13473

Print datasheet

Synonyms

HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

验证数据展示

  • IF Staining of HepG2 using CL488-13473

    IF Staining of HepG2 using CL488-13473

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HRD1/SYVN1 antibody (CL488-13473) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13473-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13473 targets HRD1/SYVN1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen HRD1/SYVN1 fusion protein Ag4267 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synovial apoptosis inhibitor 1, synoviolin
Synonyms HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68-76 kDa
GenBank Accession NumberBC030530
Gene Symbol SYVN1
Gene ID (NCBI) 84447
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TM6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HRD1/SYVN1 antibody CL488-13473Download protocol
Standard Protocols
Click here to view our Standard Protocols