验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13473 targets HRD1/SYVN1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | HRD1/SYVN1 fusion protein Ag4267 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | synovial apoptosis inhibitor 1, synoviolin |
| Synonyms | HRD1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1 |
| Calculated Molecular Weight | 617 aa, 68 kDa |
| Observed Molecular Weight | 68-76 kDa |
| GenBank Accession Number | BC030530 |
| Gene Symbol | HRD1 |
| Gene ID (NCBI) | 84447 |
| RRID | AB_3083941 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TM6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HRD1/SYVN1 antibody CL488-13473 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |