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验证数据展示

  • IF Staining of mouse pancreas using CL647-17785

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • FC experiment of PC-12 using CL647-17785

    FC experiment of PC-12 using CL647-17785

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Synaptophysin (CL647-17785) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse pancreas using CL647-17785

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 647-conjugated Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-17785

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

SYP, SYN, MRX96

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue, mouse brain tissue
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17785-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17785 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Synaptophysin fusion protein Ag11781 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptophysin
别名 SYP, SYN, MRX96
计算分子量 313 aa, 34 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC064550
基因名称 Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920235
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08247
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-17785Download protocol
Standard Protocols
Click here to view our Standard Protocols