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验证数据展示

  • IF Staining of mouse pancreas using CL647-17785

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200.

  • FC experiment of PC-12 using CL647-17785

    FC experiment of PC-12 using CL647-17785

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Synaptophysin (CL647-17785) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse pancreas using CL647-17785

    IF Staining of mouse pancreas using CL647-17785

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CoraLite® Plus 647 Synaptophysin antibody (CL647-17785) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 647-conjugated Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL647-17785

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

SYP, SYN, MRX96

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue, mouse brain tissue
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy Synaptophysin Ready-To-Use IHC Kit. Synaptophysin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17785-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17785 targets Synaptophysin in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Synaptophysin fusion protein Ag11781 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptophysin
Synonyms SYP, SYN, MRX96
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC064550
Gene Symbol Synaptophysin
Gene ID (NCBI) 6855
RRIDAB_2920235
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08247
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Synaptophysin antibody CL647-17785Download protocol
Standard Protocols
Click here to view our Standard Protocols